Determination of MIC (A) and MBC (B) of Br-CA against <i>E</i>. <i>coli</i>.

<p>MIC was defined as the lowest concentration of Br-CA to completely inhibit cell growth at 37°C in 96 wells plates for 24 hour and OD600 was monitored with the microplate reader to indicate the cell growth. MBC was defined as the lowest concentration of Br-CA to completely eradicate all the cells incubated at 37°C in 96 wells plates for 24 hour and surviving cells were evaluated using drop plating method after serial dilutions. ID stood for the initial cell density. Each data point or bar was indicated as the means of three replicates ± standard deviation.