Generation and characterization of interferon-lambda 1-resistant H1N1 influenza A viruses - Fig 6

<p><b>(A, B, C) Influenza-induced IFN gene expression levels in Calu-3 cells.</b> Cells were infected with the indicated recombinant viruses (MOI = 1) and the levels of IFNs were quantified by qPCR at 12, 48, and 72 hpi. Values were determined by comparison to standard curves for each gene and the results are expressed as RNA copy numbers. *<i>P</i> < 0.05; °<i>P</i> < 0.01, compared to the values for the CA/04 virus. <b>(D, E) IFN-λ1 and –λ2/3 protein production in Calu-3 cells.</b> Cells were infected with viruses at a MOI of 5. Supernatants were collected at 24, 48, and 72 hpi, and the levels of secreted IFN-λ1 protein were determined by ELISA. °<i>P</i> < 0.01, compared to the values for the CA/04 virus.