Tn-Seq profiles for mecillinam resistance loci.

<p>Shown are profiles of transposon insertions mapped by Tn-Seq for MG1655/pTB63 [WT/ftsZ^up] transposon libraries grown without treatment (no drug) or harvested following growth on agar containing 1 or 10 μg/ml mecillinam (low and high MEC, respectively). In the profiles, the reading frames are indicated at the bottom of the panel. Transposon insertion sites are indicated by lines above the reading frames with their height reflecting the number of reads for each insertion. A. Profiles for the known mecillinam-resistance loci: mrdAB (pbp2 rodA), mreBCD, and slt. Note that mutants defective in mrdAB (pbp2 rodA), or mreBCD have a slow growth phenotype even in FtsZ^up cells such that insertions in these genes are underrepresented in the original library B. Profiles for novel mecillinam-resistance loci: waaQ-P, ptsHI, and sspA. Note that the profiles from no drug samples are displayed with a 200 read maximum (upper) and a 20,000 read maximum (lower) to show both the overall transposon insertion distribution in the original library and the magnitude of enrichment observed in the drug treated samples. A complete list of resistance loci and their enrichment ratios (comparing total reads for all insertions with and without drug) is given in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006934#pgen.1006934.s004" target="_blank">S1 Table</a>.