Bacterial ExoU is associated with increased neutrophil transepithelial migration and LTB₄ generation.

<p>Inverted H292 monolayers were infected with various doses of matched strains of either (A, C) PAO1 or (B, D) PA14 that express or lack ExoU. Neutrophils were provided to the basolateral surface and allowed to migrate. Neutrophil migration was reported as a percent of Control. The magnitude of the neutrophil migration response to parental strains PAO1 Vector or PA14 at infection doses of 1.59E+07 and 1.34E+09 respectively were considered the Control and set at 100%. We assayed (A, B) total neutrophil migration, as well as (C, D) total LTB₄ in the apical space following 2h migration. Data are shown as mean +/- SD, and are representative of multiple independent experiments. *p ≤ 0.05, **p < 0.01, ***p < 0.001 indicate comparison between ExoU lacking or expressing genetically matched strains at the same concentration.