Increased number of senescence associated β-galactosidase (SA-β-gal)-positive cardiomyocytes and amount of lipofuscin in aging and treated cells.

<p>(A) Cardiomyocytes were incubated with 10⁻⁵ mol/L ISO for 48h. Cells were then stained for the presence of SA-β-gal as described in the Methods. Data are means ± SEM (n = 3; *P < 0.05 <i>vs</i>. vehicle). (B) The percentage of SA-β-gal-positive cells was calculated. (C) Frozen heart tissue sections were analyzed for SA-β-gal staining, and (D) the number of SA-gal-positive cells was counted. (E), Frozen heart tissue sections were analyzed for lipofuscin and (F) the percentage of lipofuscin-positive cells was calculated. Data are means ± SEM (n = 6; ***P < 0.001 vs.vehicle group; ###P < 0.001 <i>vs</i>. 2 months.).