Pathological cardiac hypertrophy induction.

<p>(A) HW/BW and HW/TL ratio in ISO-treated rats and controls (B) The gene expression of ANP was examined with the use of quantitative RT-PCR. (C) Cardiomyocyte area was evaluated by H&E staining and quantified. (D) Wall thickness was measured from M-mode tracings as described in the methods and calculated. (E) The percent area of myocardial fibrosis was examined by picric-sirius red staining. Data are means ± SEM (n = 6; **P < 0.01, ***P < 0.001 <i>vs</i>. vehicle group).