Minimum inhibitory concentration (MIC) time courses of adaptive evolution.

<p>Plots show the MICs of the treatments to the 3 drugs and lysogeny broth (LB) over time. The top (A, B, C), middle (D, E, F), and bottom (G, H, I) rows show the MICs to piperacillin (PIP), tobramycin (TOB), and ciprofloxacin (CIP), respectively. The first, second, and third columns show the MICs of the PIP^R, TOB^R, and CIP^R lineages, respectively. The dotted black lines mark the Day 20 MICs of the 3 drugs (i.e., MIC_PIP of Day 20 PIP^R in (A), MIC_TOB of Day 20 TOB^R in (E), and MIC_CIP of Day 20 CIP^R in (I)). Error bars show SEM of 4 replicates per treatment. See <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2001586#pbio.2001586.s018" target="_blank">S3 Data</a> for the raw data.