Functional assessment of liver-resident CD49a+ CD25 ihNK cells.

<p><b>(A)</b> Representative histograms 7 days post-cell tracing with CFSE on 5 livers, LTXHH24, LTXHH26, LTXHH29, LTXHH30 and LTXHH32 (gated on live cells). Intrahepatic NK cells were sorted using a BD FACSARIA FUSION according to their expression levels of CD49a and CD25. Recovered cells were CFSE-cell stained and cultured for 7 days with low amounts of IL-2 (red: CD49a+CD25+, blue: CD49a+CD25-, orange: CD49a-CD25+, green: CD49a-CD25-). <b>(B)</b> Summary of the cell proliferation data from the four sorted populations. Percentage of CFSE+ cells was determined by setting the gate on the lower part of the CD49a-CD25- population (in green) as shown by the dashed lines. Data in <b>(B)</b> is depicted as scatter plot showing all individuals, the bar represents the median and the deviation is depicted as interquartile range.