Successful application of global fitting analysis of hemoglobin physiological datasets.

<p>(A) Global fitting analysis of the human hemoglobin pH, CO₂ and 2,3-BPG physiological datasets using the traditional form of the MWC equation. Source data is indicated above each panel. (B) Dependence of observed (open circles) and calculated (filled circles) Hill values of each dataset on effector concentration. (C) Dependence of the apparent <i>L</i> values of each physiological dataset on effector concentration. Solid curves represent the results of curve fitting to the MWC-derived equation , assuming non-exclusive binding of the inhibitor effector (I) to both the <b>T</b> and <b>R</b> MWC conformations [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182871#pone.0182871.ref011" target="_blank">11</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182871#pone.0182871.ref012" target="_blank">12</a>]. In the case of organophosphate inhibitors, a power of one was used in the above equation, as only one site is available to BPG for binding to hemoglobin.