Analysis of apoptotic pathways.

<p><b>(a)</b> RT-PCR shows changes in transcription of p53, MDM2, p21, BID, Bax, caspase 9, and caspase 3 after 4 hours, 24 hours and 48 hours treatment with 2 mM methyl pyruvate in the presence and absence 0.5 μM irinotecan in RMG-1, A549, MDA-MB 231, and MRC-5 cells including MRC-5 cells during the recovery period. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Semi-quantitative analysis in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182789#pone.0182789.s002" target="_blank">S2 Fig</a>. <b>(b)</b> Western Blot analysis showing changes in expression of p53, RBBP6 and cytochrome c proteins in RMG1, A549, MDA-MB 231, and MRC5 cell lines after treatment as indicated. Again MRC5 cells were allowed to recovery for a further 24 hours after each treatment. Actin was used a loading control. Densitometry semi-quantitative analysis shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182789#pone.0182789.s003" target="_blank">S3 Fig</a>.