<p>(A) Southern blot analysis was performed using the genomic DNA of transformed hairy root clones. (B) RT-PCR analysis of <i>EPO</i> mRNA in hairy root cultures <sup>pK7</sup>cal/rh<i>EPO</i><sup>HR</sup> and <sup>pK7</sup>rh<i>EPO</i><sup>HR</sup>. The presence of mRNA for tobacco <i>β actin</i> gene is shown as an internal control for transcript analysis.</p
Fig. 7. (A) Identification of positive transgenic hairy roots lines by PCR (A, 35S + SmbHLH3; B, hpt...
Total RNA was extracted from leaf, root, and young and mature seed tissues and the expression levels...
<p>(A) PCR analyses of T<sub>0</sub> transgenic tobacco lines. (B) RT-PCR analyses of <i>UGT85A5</i>...
<p>Tobacco 18S was used as an internal control. Total RNA was isolated from roots. Numbers represent...
The hairy root, a specialized plant tissue that emerges from a cell transformed with transfer DNA (T...
<p>(A) RT-PCR amplification of GUS transcripts from total RNA isolated from <i>N</i>. <i>tabacum</i>...
<p>Total RNA was isolated from leaf tissues of indicated tobacco and pothos plants and subject to re...
<p>(A) Roots of <i>rhd6</i> seedlings grown for three days on unsupplemented (MS) media, and then tr...
<p>(A) Total RNA was extracted from a pool of 30–50 hair bulbs and mid-segments of hair follicle tis...
<p>PCR analysis of <i>rol</i>B (670 bp), <i>rol</i>C (534 bp), <i>aux</i>1 (350 bp) and <i>vir</i>D ...
Comparison of mean transcript levels from the analyzed T-DNAs. (A) Means of the IR transcript levels...
(A) Wild-type and empty-vector plants served as controls. RNA from 10 plants per treatment was extra...
<p>Significant differences of the means (± SD) between WT plants and <i>pds2-1</i> plants (n = 3) ar...
<p>A: bacteria-free seedlings; B: induction of hairy roots; C: subcultured hairy root; D: hairy root...
<p><b>A</b>) SAUR76 transcript abundance in roots and leaves of control plants, plants treated with ...
Fig. 7. (A) Identification of positive transgenic hairy roots lines by PCR (A, 35S + SmbHLH3; B, hpt...
Total RNA was extracted from leaf, root, and young and mature seed tissues and the expression levels...
<p>(A) PCR analyses of T<sub>0</sub> transgenic tobacco lines. (B) RT-PCR analyses of <i>UGT85A5</i>...
<p>Tobacco 18S was used as an internal control. Total RNA was isolated from roots. Numbers represent...
The hairy root, a specialized plant tissue that emerges from a cell transformed with transfer DNA (T...
<p>(A) RT-PCR amplification of GUS transcripts from total RNA isolated from <i>N</i>. <i>tabacum</i>...
<p>Total RNA was isolated from leaf tissues of indicated tobacco and pothos plants and subject to re...
<p>(A) Roots of <i>rhd6</i> seedlings grown for three days on unsupplemented (MS) media, and then tr...
<p>(A) Total RNA was extracted from a pool of 30–50 hair bulbs and mid-segments of hair follicle tis...
<p>PCR analysis of <i>rol</i>B (670 bp), <i>rol</i>C (534 bp), <i>aux</i>1 (350 bp) and <i>vir</i>D ...
Comparison of mean transcript levels from the analyzed T-DNAs. (A) Means of the IR transcript levels...
(A) Wild-type and empty-vector plants served as controls. RNA from 10 plants per treatment was extra...
<p>Significant differences of the means (± SD) between WT plants and <i>pds2-1</i> plants (n = 3) ar...
<p>A: bacteria-free seedlings; B: induction of hairy roots; C: subcultured hairy root; D: hairy root...
<p><b>A</b>) SAUR76 transcript abundance in roots and leaves of control plants, plants treated with ...
Fig. 7. (A) Identification of positive transgenic hairy roots lines by PCR (A, 35S + SmbHLH3; B, hpt...
Total RNA was extracted from leaf, root, and young and mature seed tissues and the expression levels...
<p>(A) PCR analyses of T<sub>0</sub> transgenic tobacco lines. (B) RT-PCR analyses of <i>UGT85A5</i>...