Additional file 8 of A comprehensive evaluation of the sl1p pipeline for 16S rRNA gene sequencing analysis

OTU clustering methods perform variably when all OTUs are included. As visualized in Fig. 4, the number of observed OTUs varies depending on clustering approach. Variability is also observed between sequencing and PCR replicates. OTUs not recognized as Bacteria were removed prior to analysis. (PDF 121 kb