Effects of S1P and SPHK I₂ on sphingolipid-related pathways/Quantification of western blots of HCF cell lysates following four week treatments with S1P or SPHK I₂.

<p>(A) Treatment with 5μM concentration of S1P on HCF 3D constructs led to significant downregulation (p ≤ 0.0001) of SphK1 when compared to control HCFs. (B) Treatment with 0.01μM, 0.1μM, and 5μM concentrations of S1P on HCF 3D constructs led to significant downregulation (p ≤ 0.01, p ≤ 0.0001, p ≤ 0.0001) of SphK2 when compared to controls. (C) Treatment with 0.1μM and 5μM concentration of S1P on HCF 3D constructs led to significant downregulation (p ≤ 0.001 and p ≤ 0.0001) of S1PR3 when compared to controls. (D) Treatment with 2μM, 5μM, and 10μM concentrations of SPHK I₂ on HCF 3D constructs led to significant upregulation (p ≤ 0.05) of S1PR3 when compared to controls. A one-way ANOVA was performed and p ≤ 0.05 considered statistically significant. * represents p ≤ 0.05, ** represents p ≤ 0.01, *** represents p ≤ 0.001, **** represents p ≤ 0.0001.