Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA - Fig 7

<p><b>Delayed Inhibition of Origin Firing by MMS Correlates with Delayed Checkpoint Kinase Activation</b> A) Wild-type cells with an HA-tagged Cds1 (yFS988) were G1 synchronized, released into S phase is the presence of 1μM 4NQO, 16.5 μM bleomycin or 3.5 mM MMS, harvested at the indicated times and processed for Cds1 IP kinase assays. Average signal normalized to the 4NQO 90-minute timepoint is plotted ± standard error of the mean. B) Representative kinase assays. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006958#pgen.1006958.s008" target="_blank">S8 Fig</a> for data from each replicate.