CRP2 accelerates actin filament formation and clustering.

<p>(A) Confocal laser scanning microscopy images of Alexa Fluor-labeled bare F-actin and CRP2-bound F-actin. Actin monomer (0.042 mg/mL) was incubated with or without CRP2 under filament-forming conditions for 4 h. The molar ratios of actin monomer:CRP2 were 1:0, 1:0.25, 1:0.5, and 1:1. (B) The lengths of single actin bundled filament with or without CRP2. Actin monomer (0.17 mg/mL) was incubated with or without 0.022 mg/mL CRP2 under filament-forming conditions for 4 h. The molar ratio of actin monomer:CRP2 was 1:0.25. The data shows the mean ± standard deviation (n = 40). <i>p</i> < 0.001. (C) Actin polymerization assay. Pyrene fluorescence of actin monomer (0.4 mg/mL) containing 10% pyrene-labeled was recorded under filament-forming conditions with or without CRP2. The molar ratios of actin monomer:CRP2 were 1:0, 1:0.12, 1:0.5, and 1:1.