The expression profile of <i>ipf</i> and <i>klf</i> in the mouse model.

<p>Female C57BL/6 mice (five animals per group) were infected orally with ∼8×10⁸ CFU of <i>S</i>. Infantis strains harboring luciferase reporter fusion with <i>ipf (ipf</i>::<i>lux)</i> <b>(A, C)</b> and <i>klf</i> (<i>klf</i>::<i>lux</i>) (<b>B, D</b>). Twenty four hours p.i. mice were sacrificed and their intact GI tract was removed and imaged immediately using a photon-counting <i>in-vivo</i> imaging system. (<b>A</b> and <b>B</b>) Bacterial loads in the cecum, colon and ileum are indicated by total CFU counted in the entire organ. Each dot indicates the count in an entire organ in a single animal. (<b>C</b> and <b>D</b>) The detected bioluminescence signal is shown as pseudocolor images, with variations in color representing light intensity at a given location. The color bar indicates relative signal intensity (as photons s⁻¹ cm⁻² sr⁻¹) and the minimal and maximal values measured are indicated in the box below each image. Different organs are indicated as follow: jejunum (J); ileum (I); cecum (CE); and colon (C).