Placental CD31 and CD34 protein.

<p>Protein levels of CD31 and CD34 in total placental homogenates of normal pregnancy (NP, white bar) and gestational diabetes mellitus (GDM, hatched bar) were determined by western blot. <b>A)</b> Representative western blot for CD31 (55 kDa), CD34 (80 kDa) and β-actin (43 kDa). <b>B)</b> Densitometric analysis of CD31/ β-actin ratio (left Y-axis) and CD34/ β-actin ratio (right Y-axis). <b>C)</b> Representative images of CD34 staining in placentas from Normal and GDM. <b>D)</b> Number of vessels per villi. <b>E)</b> Mean blood vessel area (MVA)/mean chorionic villi area (MCVA) ratio. In B, n = 6–12 in each group. In D and E, n = 4–5 in each group. Magnification 40X. P-value is indicated in each graph.