Mice lacking Myd88 in CD4 T cells are susceptible to multiple infections.

<p>CD4-Cre Myd88^fl/fl mice and littermate control were infected with 5×10⁵ <i>Salmonella</i> Typhimurium. Two weeks later, infected mice were injected i.v. with 10ug LPS. (A-C) Spleens were collected 4 hours later to examine IFN-γ production by Th1 cells by flow cytometry. Representative flow cytometry plots (A) and a graph of combined data (B) are shown. Serum was also collected 4 hours after LPS treatment to determine IFN-γ concentration by ELISA (C). (D-F) Bacterial loads in the spleen and/or liver of individual mice were determined at day 35 post-infection for <i>Salmonella</i>-infected mice (D), day 8 and day 11 for <i>Chlamydia</i>-infected mice (inclusion-forming units, IFC) (E), or day 9 (early stage) and day 56 (late stage) for <i>Brucella</i>-infected mice (F). Data shown were combined from two experiments containing at three to five mice per group. Statistical analyses were performed by two-way ANOVA with a Bonferroni posttest. Error bars represent the mean ±SEM. p < 0.0001 (****), 0.0002 (***), 0.0021 (**), 0.0332 (*), 0.1234 (ns).