LPAP phosphoforms detection with phospho-site-specific antibodies.

<p>CEM cells or PBMCs were activated with PMA (10 ng/ml) for 30 min or left untreated. LPAP immunoprecipitated from CEM cells (A), thymocytes (B) or PBMCs (C) was analyzed by 18% SDS-PAGE followed by Western blotting using monoclonal anti-LPAP (top) or phospho-specific mouse antisera pSer-99, pSer-153, pSer-163, and pSer-172. Samples were dephosphorylated with CIP or left untreated (indicated as + or −).