Effects of heterozygous RORγt deletion on cytokine production in post-infarct myocardium.

<p>(A) RORγt^+/- mice and WT mice were subjected to MI operation, and hearts were harvested at day 7 after MI. Total RNA was prepared from hearts from the ligation point to the apex, and quantitative RT-PCR was performed for RORγt, IL-17A, IL-17F, IL-23R IL-6, IL-1β, TNF-α, IFN-γ, IL-10 and TGF-β mRNA levels. The expressions of the transcripts were normalized with that of GAPDH and shown as the ratio to the average value of WT mice. Data are shown as means ± SD; n = 13 for WT mice, 8 for RORγt^<b>+/-</b> mice. *<i>P</i> < 0.05 and **<i>P</i> < 0.01 vs WT mice by Student’s <i>t</i>-test. (B) Heart homogenate was prepared from infarct hearts of RORγt^+/- mice and WT mice at day 7 after MI. ELISA was performed to evaluate the concentration of IL-10 protein. Data are shown as means ± SD; n = 6 for WT mice, n = 7 for RORγt+/- mice. **<i>P</i> < 0.01 vs WT mice by Student’s <i>t</i>-test.