MOESM1 of Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique

Additional file 1: Figure S1. Concentration optimization of anti-LC3 antibody and LC3 recombinant protein. (A) Anti-LC3 antibody was determined an appropriate concentration at dilution 1:20 and 1:100. Row 1 and 3, and row 2 and 4 represent to the amount of LC3 recombinant protein before and after applying into the column, respectively. Percentage of bound antibodies was estimated using ImageJ software program. (B) LC3 recombinant protein was investigated an appropriate concentration among 0.25 to 4 μg/μl