Surface markers of microparticle.

<p>BAL was double stained with annexin V and cell surface markers and analyzed by a flow cytometer: isotype control (A), CD11b (B), CD41a (C), CD45 (D), keratan sulfate (E), and SPD (F). MPs were gated using 1.33 μm beads as size markers, and the annexin V-positive gate was analyzed for the expression of the surface markers. To distinguish MPs from apoptotic bodies, BAL was double stained with annexin V and PI (G) using apoptotic bodies from hydrogen peroxide treated HT29 cells as a positive control (H). To detect surface sialic acid on MPs, BAL was double stained with annexin V and lectins. Annexin V-positive gate was analyzed for the expression of SNA (I) and MAA1 (J). The histrogram are representative of ten BAL samples.