Integrated target prediction analysis uncovers miRNA/mRNA interaction networks regulated by HSF-1 during HS.

<p>The miRNAs (rectangles) that we determined to be regulated by HSF-1 during HS were used for target prediction analysis carried out by mirWIP or TargetScan. The mRNA targets (circles) were consolidated by comparing the predicted mRNAs to those determined by mRNA-sequencing, performed in parallel to miRNA sequencing, to be regulated by HSF-1 during HS. Interactions were predicted using the Agilent literature search tool, and network generation was done with Cytoscape. Transcripts that are not colored were not affected in our dataset, but are neighbors shared by at least two transcripts that were affected. The color of each miRNA or mRNA corresponds to the degree of HSF-1 regulation, where red represents induction and blue represents suppression. Bold connecting lines represent connections between upregulated and downregulated clusters.