Localization of paracoccin and chitin in yeast cells of <i>Paracoccidioides brasiliensis</i>.

<p>After 10 days cultured at 37°C, <i>P</i>. <i>brasiliensis</i> yeasts were stained for detection of paracoccin, using chicken IgY anti-paracoccin antibody conjugated to Alexa Fluor 488 (green), and for chitin with Texas Red^®-X WGA (red). (E) Orthogonal analysis confirmed the localization of paracoccin and WGA. The orthogonal (XY, XZ and YZ) analysis was performed to confirm the localization of paracoccin and chitin in the yeasts. DNA was stained with DAPI (blue). The images are a single section from a Z series stack.