<p>A. FLAG-Fbw7α was transfected or not in HeLa cells, as indicated. Total extracts of asynchronous cells (AS), cells synchronized in G1/S or arrested in pro-metaphase and recovered by shake-off (M) were analyzed by SDS-PAGE and immunoprobed for pS18-Fbw7α and FLAG-Fbw7α. The migration of Cdc27 was used as a marker of M phase (Cdc27<sup>P</sup>), and anti-tubulin as a loading control. B. HeLa cell extracts (500 μg) from cells arrested in prometaphase were subjected to immunoprecipitation with anti-Fbw7 or control antibodies and analyzed by immunoblotting to detect phosphorylated Ser18 (pS18). Input 10% (i), supernatant after IP (s). C. HeLa cells grown on coverslips were co-transfected with FLAG-Fbw7α and either GFP- PKCα, PKCβ1, PKCε or PK...
<p>(A) HEK293T cells transfected with FLAG-tagged GFP, CEP215 (WT) and CEP215<sup>F75A</sup> (F75A) ...
<p>(<b>A</b>) Asynchronous HeLa cells stained with antibodies against FOP (green) and PCM-1 (red) sh...
<p>(A) <i>In vitro</i> interaction between TBC1D3 and Fbw8. Separate sets of HeLa cells were transfe...
(A) Left panel: FBW7α and γ reduces WDR62 protein level. HEK293 cells were transfected as indicated;...
<p>(A) Combination of endogenous GFP fluorescence and anti-FLAG-immunostaining under fixed or live c...
<p>(A) Dephosphorylation of Gwl and SP sites in B55 depleted cells. HeLa cells were transfected with...
<p>(A–C) Representative images of HeLa cells transfected with PKCε-EGFP and mCherry (A), A10-mCherry...
<p>HeLa cells were transfected with HA-CUL7, Flag-TBC1D3 and Myc-Fbw8 constructs. At 18 h after tran...
<p>(A–F) <i>cdc15-2</i> (F1492), <i>cdc13-1 cdc15-2</i> (F1488), <i>cdc13-1 rad53-21 cdc15-2</i> (F1...
<p>(<b>A</b>) Hela expressing Gag-GFP cells were transfected with indicated siRNAs. Culture supernat...
<p>A) U87MG cells transfected with the control or with siPKCιA and siPKCιB were transiently transfec...
<p>A) HEK293T cells were transfected with 3X-FLAG tagged plasmids encoding IKKγ, TRAF3, TRAF6, IKKε,...
(A) Potential pUs3 substrates, indicated in red, reside in HSV-2 pUL31 (top) and in HSV-2 pUL34 (bot...
<p>A. Fbxw4 can be immunoprecipitated by ubiquitinated proteins and Fbxw4 can immunoprecipitate ubiq...
<p>A. Table representing the number of unique peptides identified from one representative mass spect...
<p>(A) HEK293T cells transfected with FLAG-tagged GFP, CEP215 (WT) and CEP215<sup>F75A</sup> (F75A) ...
<p>(<b>A</b>) Asynchronous HeLa cells stained with antibodies against FOP (green) and PCM-1 (red) sh...
<p>(A) <i>In vitro</i> interaction between TBC1D3 and Fbw8. Separate sets of HeLa cells were transfe...
(A) Left panel: FBW7α and γ reduces WDR62 protein level. HEK293 cells were transfected as indicated;...
<p>(A) Combination of endogenous GFP fluorescence and anti-FLAG-immunostaining under fixed or live c...
<p>(A) Dephosphorylation of Gwl and SP sites in B55 depleted cells. HeLa cells were transfected with...
<p>(A–C) Representative images of HeLa cells transfected with PKCε-EGFP and mCherry (A), A10-mCherry...
<p>HeLa cells were transfected with HA-CUL7, Flag-TBC1D3 and Myc-Fbw8 constructs. At 18 h after tran...
<p>(A–F) <i>cdc15-2</i> (F1492), <i>cdc13-1 cdc15-2</i> (F1488), <i>cdc13-1 rad53-21 cdc15-2</i> (F1...
<p>(<b>A</b>) Hela expressing Gag-GFP cells were transfected with indicated siRNAs. Culture supernat...
<p>A) U87MG cells transfected with the control or with siPKCιA and siPKCιB were transiently transfec...
<p>A) HEK293T cells were transfected with 3X-FLAG tagged plasmids encoding IKKγ, TRAF3, TRAF6, IKKε,...
(A) Potential pUs3 substrates, indicated in red, reside in HSV-2 pUL31 (top) and in HSV-2 pUL34 (bot...
<p>A. Fbxw4 can be immunoprecipitated by ubiquitinated proteins and Fbxw4 can immunoprecipitate ubiq...
<p>A. Table representing the number of unique peptides identified from one representative mass spect...
<p>(A) HEK293T cells transfected with FLAG-tagged GFP, CEP215 (WT) and CEP215<sup>F75A</sup> (F75A) ...
<p>(<b>A</b>) Asynchronous HeLa cells stained with antibodies against FOP (green) and PCM-1 (red) sh...
<p>(A) <i>In vitro</i> interaction between TBC1D3 and Fbw8. Separate sets of HeLa cells were transfe...