Induction of RAD21 degradation and KSHV lytic cycle by protein synthesis inhibitors in BCBLI cells.

<p><b>(A)</b> Immunoblotting of RAD21 in KSHV latently infected BCBLI cells exposed to different antibiotics. BCBLI cells were incubated with 10ug/ml Emetine, 75ug/ml Puromycin, 0.2 ug/ml Pactamycin, 10ug/ml Anisomycin or 100 ug/ml cycloheximide for 0, 2, 4, 6 and 8 hours. <b>(B-F)</b> RT-qPCR for ORF45-50, LANA and RAD21 transcripts relative to cellular 18S in BCBLI cells treated for 0–8 hrs with Emetine (EME), Puromycin (Puro), Pactamycin (Pact), Anisomycin (Anis) or Cycloheximide (CHX) as described in (A).