<p>Comparison of the PCR amplification rates of a fusion <i>Neq</i>SSB<i>-TaqS</i> DNA polymerase for 300 bp (A), 500 bp (B), 1000 bp (C) products and a <i>Taq</i>S DNA polymerase for 300 bp (D), 500 bp (E), 1000 bp (F) products. The elongation times used for the PCR amplification are indicated at the top. Lane M: the DNA molecular size marker (50–2000 bp).</p
<p>(A) Agarose gel (2.0%) electrophoresis indicates amplification of a single PCR product of the exp...
pre-printBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide i...
"nFluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantific...
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology...
<p>*PCR conditions: denature at 94°C for 3 min, 1 cycle; denature at 94°C for 1 min, anneal at 57°C ...
<p>n = sample size. Each extraction method was tested six times on each material. PCR and qPCR were ...
Forensic genotyping uses a multiplex short tandem repeat (STR) assay to amplify deoxyribonucleic aci...
DNA amplification is a very powerful tool that can be used to replicate one molecule of DNA into mil...
Quantitative real-time RT-PCR is the most sensitive and widely used method for the measurement of ge...
<p>(A) PCR verification of expected 199 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecul...
<p>Square fields indicate amplification of the ~350 bp fragment with different intensity. Black fiel...
<p>(A) A density plot of the expected distribution of fragment lengths, with peaks at 124 and 142 bp...
Currently, the amplification step of most forensic DNA profiling systems takes 3–4\ua0h to complete....
BACKGROUND: PCR is a key technology in molecular bi-ology and diagnostics that typically amplifies a...
<p>(a) Primer and template sets. WT and Mu indicate wild-type and mutant types, respectively. The 3'...
<p>(A) Agarose gel (2.0%) electrophoresis indicates amplification of a single PCR product of the exp...
pre-printBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide i...
"nFluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantific...
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology...
<p>*PCR conditions: denature at 94°C for 3 min, 1 cycle; denature at 94°C for 1 min, anneal at 57°C ...
<p>n = sample size. Each extraction method was tested six times on each material. PCR and qPCR were ...
Forensic genotyping uses a multiplex short tandem repeat (STR) assay to amplify deoxyribonucleic aci...
DNA amplification is a very powerful tool that can be used to replicate one molecule of DNA into mil...
Quantitative real-time RT-PCR is the most sensitive and widely used method for the measurement of ge...
<p>(A) PCR verification of expected 199 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecul...
<p>Square fields indicate amplification of the ~350 bp fragment with different intensity. Black fiel...
<p>(A) A density plot of the expected distribution of fragment lengths, with peaks at 124 and 142 bp...
Currently, the amplification step of most forensic DNA profiling systems takes 3–4\ua0h to complete....
BACKGROUND: PCR is a key technology in molecular bi-ology and diagnostics that typically amplifies a...
<p>(a) Primer and template sets. WT and Mu indicate wild-type and mutant types, respectively. The 3'...
<p>(A) Agarose gel (2.0%) electrophoresis indicates amplification of a single PCR product of the exp...
pre-printBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide i...
"nFluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantific...