Clonogenic assays.
- Anna Maria Lustri (380641)
- Sabina Di Matteo (827248)
- Alice Fraveto (827242)
- Daniele Costantini (827247)
- Alfredo Cantafora (4413427)
- Chiara Napoletano (765803)
- Maria Consiglia Bragazzi (827243)
- Felice Giuliante (823874)
- Agostino M. De Rose (4413421)
- Pasquale B. Berloco (4413424)
- Gian Luca Grazi (827245)
- Guido Carpino (77837)
- Domenico Alvaro (77827)
Publication date
September 2017
DOIAbstract
<p>The number of colonies (A and B) and the absorbance of the extracted dye (crystal violet) measured at 595 nm (C) indicate the clonogenic potential of CCA cell cultures. We tested the clonogenic capacity of mucin-IHCCA cells after sequential treatment with CX4945 (72 hrs) and MK2206 (48 hrs) versus their combination. The sequential treatment of CX4945 followed by MK2206 showed the highest inhibitory effect on mucin-IHCCA cell growth. We calculated the numbers of colonies and the absorbance of the extracted dye at 595 nm. & = p<0.05 vs non pre-incubated conditions. $ = p< 0.05 vs others columns. Mean +/- SD of N = 3–5 independent experiments.</p
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