ER-stress induced by Thapsigargin, Tunicamycin or DTT is not attenuated by ALR.

<p>Cultured cells were incubated either for 6 hours with 0.5 μM Thapsigargin, for 16 hours with 10 μg/ml Tunicamycin or 2 mM DTT. (A) Activation of CHOP was analyzed by western blotting of HepG2-sfALR and HepG2 cells treated without or with 100 nM rhALR. (B) Activation of XBP1 in HepG2-sfALR and HepG2 cells treated without or with 100 nM rhALR was analyzed by amplifying XBP1 cDNA by PCR followed by incubation with P<i>st</i>I. Spliced form indicating activation results in a 473 bp amplification product, whereas unspliced form of XBP1 shows digested 290 bp and 183 bp amplification products (one out of three experiments is shown).