Bisulfite sequencing analysis to confirm the RT-MSP data.

<p>The results of bisulfite sequencing of the 5' <i>IRF6</i> CGI for representative samples are shown. The clinical melanoma samples #10 and #17 were examined as representatives of samples with high methylation levels. Normal cultured melanocytes MC2 and clinical melanocytic nevus samples #23 and #34 were examined as representatives of samples with low methylation levels. Closed and open circles indicate methylated and unmethylated CpG sites, respectively. Closed and open triangles indicate the location of the RT-MSP primer sets specific to the methylated and unmethylated DNA sequences, respectively. The vertical and horizontal numbered rows indicate each clone and CpG site, respectively.