Concordance of the methylation status of the 5' <i>IRF6</i> CGI with <i>IRF6</i> transcription or IFN-β sensitivity in melanocyte-lineage cells.

<p>The left bar graph indicates the methylation levels (%) of the 5' <i>IRF6</i> CGI in the indicated melanoma cell lines, normal cultured melanocytes (MC1 and MC2) and controls including methylated DNA (M) and unmethylated DNA (U). The second bar graph from the left indicates the expression levels of <i>IRF6</i> divided by the expression levels of <i>GAPDH</i> (×10⁻⁴) in each of the melanoma cell lines and normal cultured melanocytes (MC1 and MC2) that were not treated with IFN-β. The expression level of MC1 was 1.1×10⁻³, which is higher than the 3.0×10⁻⁴ upper limit of the graph. The third bar graph from the left indicates the expression levels of <i>IRF6</i> divided by the expression levels of <i>GAPDH</i> (×10⁻⁴) in each of the melanoma cell lines treated with IFN-β. The fourth bar graph from the left indicates the cell growth rate which was defined as the value of the number of IFN-β-treated cells divided by the number of PBS-treated cells. All four bar graphs correspond to each other with respect to the melanoma cell lines and normal cultured melanocytes.