Extracted ion chromatograms of the <i>N</i>-glycan distribution at Asn52 for Bemfola and GONAL-f.

<p>Fig was constructed using MassLynx 4.1 (Waters, Milford MA, USA) from the respective XIC of the glycopeptides extracted from only Asn52. Asn, asparagine. Blue square, GlcNAc. Green circle, mannose. Yellow circle, galactose. Red triangle. fucose. Purple diamond, sialic acid NeuNAc. Glycan naming: F at the start of the abbreviation indicates a core a(1–6) fucose linked to the inner GlcNAc. Ax indicates the number of antenna (GlcNAc) on trimannosyl core. A2 indicates bi-antennary with both GlcNAcs as b(1–2) linked. A3 indicates tri-antennary with a GlcNAc linked b1-2 to both mannose and a third GlcNAc linked b(1–4) to the a(1–3) linked mannose. A4 indicates tetra-antennary with GlcNAcs linked as A3 with additional GlcNAc b(1–6) linked to a(1–6) mannose. Gx indicates the number (x) of b1-4 linked galactose on the antenna. Sx indicates the number (x) of sialic acids linked to galactose.