Point mutations in a repetitive motif in μ2 region, 283 to 325, abrogate MT-association.

<p>(A) Schematic representation of the amino acidic sequence in T1L μ2 region 283 to 325. The Asp in each Val-Asp-Val motif is underlined. In the scheme, the μ2 is fused at the C-terminus to an HA tag. (B) Confocal immunofluorescence of CV-1 cells expressing μ2-HA wild type or containing the specified point mutation. At 20 hpt, cells were methanol fixed and immunostained for detection of μ2 (mouse mAb anti-HA, green) (right upper panel) and MTs (specific polyclonal anti-alpha tubulin serum, red)(right lower panel). A Merged image is shown at the left of each panel. Nuclei are stained with DAPI (blue). Scale bar is 20 μm.