CD11c-specific HIF-1α-knockout mice produce more monocyte’s progenitors and display enhanced output of inflammatory monocytes in the bone marrow.

<p><i>Hif</i>^{<i>flox/flox</i>}-<i>Cd11c</i>-<i>Cre</i>^<i>+</i> and <i>Cre</i>^<i>-</i> mice were infected with <i>L</i>. <i>donovani</i> and sacrificed at various time point of infection. <b>(A)</b> Gating strategy to identify GMPs in the bone marrow of <i>L</i>.<i>donovani</i> infected mice. <b>(B)</b> Frequency (left graph) and absolute numbers (right graph) of GMPs in the bone marrow of mice over the course of infection. <b>(C-E)</b> Frequency (upper graph) and absolute numbers (lower graph) of granulocytes <b>(C)</b>, Ly6C^hi monocytes<b>(D),</b> Ly6C^lo/int monocytes <b>(E)</b> in the bone marrow of infected mice. <b>(F-G)</b> Percentage of CCR2⁺ <b>(F)</b> and CXCR4⁺ <b>(G)</b> Ly6C^hi monocytes in the bone marrow. <b>(H)</b> Percentage of Sca-1⁺ Ly6C^hi monocytes in the bone marrow. <b>(I)</b> Percentage of MHCII⁺ Ly6C^hi monocytes in the bone marrow. All data represent mean ± SEM of one of 4 independent experiments, n = 4. * denotes <i>p</i><0.05.