MOESM1 of Sustaining fermentation in high-gravity ethanol production by feeding yeast to a temperature-profiled multifeed simultaneous saccharification and co-fermentation of wheat straw

Additional file 1: Figure S1. Ethanol inhibition in high-gravity enzymatic hydrolysis with enzyme preparation Cellic Ctec 2. Figure S2. Shake flask SSCF with the non-flocculating strain KE6-12.A and the flocculating strain KE-Flow. Figure S3. Spotting assay of the B-Flow cells. Figure S4. Multi-feed SSCF at constant temperature or with temperature reduction. Figure S5. Investigation of medium compositions for batch and fed-batch propagation of KE-Flow with material M2. Figure S6. Adapted multi-feed SSCF with faster feeding and maximum use of pretreatment liquor. Figure S7. Comparison of cultivation of KE-Flow in 3.6 L laboratory reactors and in the demonstration plant. Figure S8. Multi-feed SSCF of material M3 in 10 m3 and 30 L reactors with KE-Flow. Table S1. SSCF feeding schemes for laboratory scale reactors. Table S2. Summary of results from multi-feed SSCF experiments using steam pre-treated wheat straw