Clusterin expression is regulated by CCK2R signaling and suppression of gastric acid secretion.

<p>(A, B, C) HDC protein level (A) and CLU protein level (B) in whole mucosa lysates by targeted mass spectrometry and plasma (C) by ELISA from control (Ctr) (n = 9), PPI-induced hypergastrinemic (PPI) (n = 6), CCK2R-antagonized (CCK2Ra) (n = 5–8) and CCK2R-antagonized PPI-induced hypergastrinemic (PPI+CCK2Ra) (n = 6–8) rats. Each dot represents an individual animal and the black lines mark median. (D) ISH (n = 4) and IHC (n = 8) of oxyntic mucosa from CCK2R-antagonized rats showing expression of clusterin (brown). (E) ISH (n = 5) and IHC (n = 8) of oxyntic mucosa from CCK2R-antagonized hypergastrinemic PPI-rats showing expression of clusterin (brown) (compare (D) and (E) to control and hypergastrinemic PPI-rats in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184514#pone.0184514.g002" target="_blank">Fig 2A</a>). (F-H) Number per oxyntic gland of (F) CLU-positive cells in IHC-stained sections (n = 5–8 rats per group), (G) dual CLU-positive/PGA5-positive cells (n = 4 rats per group), and (H) dual CLU-positive/PCNA-positive cells (n = 4 rats per group). Data presented as means ± SEM. *ANOVA with Tukey-adjusted <i>p</i> value < 0.05. Nuclei were counterstained with hematoxylin (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = 100 μm.