Replication of differentially expressed miRNAs after TGF-β1 stimulation using qRT-PCR.
- Jennie Ong (532428)
- Wim Timens (41871)
- Vijay Rajendran (4436473)
- Arjan Algra (4436470)
- Avrum Spira (5655)
- Marc E. Lenburg (265511)
- Joshua D. Campbell (615868)
- Maarten van den Berge (116456)
- Dirkje S. Postma (101740)
- Anke van den Berg (80131)
- Joost Kluiver (188970)
- Corry-Anke Brandsma (41865)
Publication date
September 2017
DOIAbstract
<p>To replicate the TGF-β1-induced expression changes of the validated miRNAs, qRT-PCR was performed on the control and COPD fibroblasts in the replication group (A) stimulated with 2.5 ng/ml TGF-β1 and (B) stimulated with 7.5 ng/ml TGF-β1. Data are presented as relative expression (2<sup>-ΔCp</sup>) normalized to RNU48. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.</p
Add to ORKG