<p>Cells given pre-treatment at 1 h after isolation until 17 h, and then were washed, suspended in fresh media, and given a further treatment (challenge) for 60 min. Quantitative data are expressed as relative density units, using beta-actin as a loading control. Naïve conditions (media only / 100 ng/mL LPS) and impaired (10 ng/mL LPS / 100 ng/mL LPS) were compared by paired T-test. N = 6. Mean ± S.E.M. *P < 0.05.</p
<p>(a) Expression of MEF2C and β-actin determined by western blot analysis. (b) The number of viable...
<p>(A) A549 cells were transfected with either pORF3-EGFP (ORF3) or pEGFP-N1 (GFP) for 48 h, stimula...
<p><b>A</b>) Naïve and resistant RAW 264.7 cells (ResRAW) were exposed to PAO1 (MOI 10) or to LPS (1...
<p>Western blot analysis of NFκB members p65 and IκBα in non-differentiated (St-T1, first line; KdS1...
<p>Cells were treated with PBS (1), 5 nmol/L rPVL for 2 h (2) or 100 nmol/L rPVL for 2 h (3), respec...
<p>(A) Don Q cells were treated without (control) or with CpPLC at different times (3, 5 or 7 hours)...
<p>(A) The NF-κB p65 protein expression was measured by Western blot in the nuclear extracts of PBMC...
<p>(<b>A</b>) hADSCs were treated with or without LPS (50 ng/ml), immediately followed by LPLI (8 J/...
<p>(<b>A</b>) Immunoblot analysis showing the expression of NF-κB/p65 (65 kDa) in tissue lysates of ...
<p>(A) The total and phosphorylation levels of NF-κB p65 was analyzed by Western blot in ICB and ret...
<p>Cells were treated with PBS (1), 5 nmol/L rPVL for 2 h (2) or 100 nmol/L rPVL for 2 h (3) respect...
<p>(A) Gel images of the western blot analyses of the p65 protein extracted in cytoplasmic and nucle...
A) Western blot assays performed on U87 cell lysates in the control condition and after treatment wi...
<p>(A) RAW264.7 cells pretreated with or without complex <b>5</b> for 4 h were treated with LPS for ...
<p>Note: Compared with NC group **: P<0.01; compared with DN0 group ##: P<0.01.</p
<p>(a) Expression of MEF2C and β-actin determined by western blot analysis. (b) The number of viable...
<p>(A) A549 cells were transfected with either pORF3-EGFP (ORF3) or pEGFP-N1 (GFP) for 48 h, stimula...
<p><b>A</b>) Naïve and resistant RAW 264.7 cells (ResRAW) were exposed to PAO1 (MOI 10) or to LPS (1...
<p>Western blot analysis of NFκB members p65 and IκBα in non-differentiated (St-T1, first line; KdS1...
<p>Cells were treated with PBS (1), 5 nmol/L rPVL for 2 h (2) or 100 nmol/L rPVL for 2 h (3), respec...
<p>(A) Don Q cells were treated without (control) or with CpPLC at different times (3, 5 or 7 hours)...
<p>(A) The NF-κB p65 protein expression was measured by Western blot in the nuclear extracts of PBMC...
<p>(<b>A</b>) hADSCs were treated with or without LPS (50 ng/ml), immediately followed by LPLI (8 J/...
<p>(<b>A</b>) Immunoblot analysis showing the expression of NF-κB/p65 (65 kDa) in tissue lysates of ...
<p>(A) The total and phosphorylation levels of NF-κB p65 was analyzed by Western blot in ICB and ret...
<p>Cells were treated with PBS (1), 5 nmol/L rPVL for 2 h (2) or 100 nmol/L rPVL for 2 h (3) respect...
<p>(A) Gel images of the western blot analyses of the p65 protein extracted in cytoplasmic and nucle...
A) Western blot assays performed on U87 cell lysates in the control condition and after treatment wi...
<p>(A) RAW264.7 cells pretreated with or without complex <b>5</b> for 4 h were treated with LPS for ...
<p>Note: Compared with NC group **: P<0.01; compared with DN0 group ##: P<0.01.</p
<p>(a) Expression of MEF2C and β-actin determined by western blot analysis. (b) The number of viable...
<p>(A) A549 cells were transfected with either pORF3-EGFP (ORF3) or pEGFP-N1 (GFP) for 48 h, stimula...
<p><b>A</b>) Naïve and resistant RAW 264.7 cells (ResRAW) were exposed to PAO1 (MOI 10) or to LPS (1...