IκK-16 decreases miRNA-155 expression and attenuates the human monocyte inflammatory response - Fig 1

<p>(A) Supernatant TNF-α concentrations and (B) IL-10 concentrations comparing naïve (no LPS pre-treatment) and impaired (10 ng/mL LPS pre-treatment) conditions. Time points are shown for the end of the pre-treatment prior to the LPS challenge (17 h), and then the response 12 h after the LPS 100 ng/mL challenge (29 h). Levels were determined by ELISA. N = 8. Mean ± S.E.M. are shown. *P < 0.05, paired T-test. Time course of TNF-α (C) and IL-10 (D) gene expression in the impaired monocyte. Monocytes were treated with 10 ng/mL of LPS given after 1h for a duration of 16 h. At 17 h, cells were washed and resuspended in fresh media, then received 100 ng/mL of LPS at 17 h. Levels were measured by qRT-PCR, and expressed as fold change (2^-ΔΔCT) relative to the naïve monocyte. Mean ± S.E.M. are shown. N = 4. *P < 0.05. # P = 0.09, paired T-test.