Tissue-specific expression of <i>trio</i> rescues pupation in the <i>seq</i>-enhanced <i>trio</i> mutant background.

<p>The percent of expected pupae was determined for the following genotypes: <i>trio</i>-mutant, <i>trio</i>-mutant background <i>(trio</i>^<i>M89</i> <i>/ trio</i>^<i>P1</i><i>); seq</i>-enhanced, <i>seq</i>-enhanced <i>trio</i> mutants (<i>seq</i>^{<i>9</i>.<i>17</i>} <i>/ +; trio</i>^<i>M89</i> <i>/ trio</i>^<i>P1</i><i>);</i> R-pan neural, rescue by pan-neural <i>trio</i> expression in the <i>seq</i>-enhanced <i>trio</i> mutant background (<i>GAL4-elav</i>.<i>L</i> / <i>seq</i>^{<i>9</i>.<i>17</i>}<i>; trio</i>^<i>M89</i> <i>/ trio</i>^<i>P1</i>, <i>UAS-trio</i>.<i>B</i>); R-class IV da, rescue by class IV da <i>trio</i> expression in the <i>seq</i>-enhanced <i>trio</i> mutant background (<i>GAL4-ppk1</i>.<i>9</i>, <i>UAS-mCD8GFP</i> / <i>seq</i>^{<i>9</i>.<i>17</i>}<i>; trio</i>^<i>M89</i> <i>/ trio</i>^<i>P1</i>, <i>UAS-trio</i>.<i>B)</i>. N = 15 for all genotypes. ANOVA p-value <0.0001; *** indicates pair-wise statistical significance at p<0.001 as compared to *seq*-enhanced. R-pan neural and R-class IV da are also statistically different from each other at p<0.001. Error bars represent the standard error of the means.