Inhibition of glucose metabolism impaired <i>in vivo</i> responses to systemic <i>C</i>. <i>albicans</i> infection.

<p>(A) Fungal burden measured in the kidneys of C57BL/6 mice treated with PBS, 2-DG or BPTES during 5 days after i.v. <i>C</i>. <i>albicans</i> challenge (mean ± SEM, n = 6; similar results were obtained in 2 independent experiments). *p<0.05, Student’s t test. Each dot represents one mouse. (B) Candidacidal activity of neutrophils isolated from blood of C57BL/6 mice treated with PBS, 2-DG or BPTES during 5 days after i.v. <i>C</i>. <i>albicans</i> challenge (mean ± SEM, n = 6; similar results were obtained in 2 independent experiments) *p < 0.05, Student’s t test. Each dot represents one mouse. (C) IL-1β, IL-6, IL-10, IFNγ and TNFα production by mouse splenocytes obtained from PBS, 2-DG or BPTES-treated mice 5 days after <i>C</i>. <i>albicans</i> i.v. infection were measured by ELISA 48 h after <i>in vitro</i> restimulation with medium, LPS, heat-killed <i>C</i>. <i>albicans</i> yeast or heat-killed <i>C</i>. <i>albicans</i> hyphae (mean ± SEM, n = 6; similar results were obtained in 2 independent experiments) *p<0.05, Student’s t test.