Additional file 3: Figure S1. of SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes
- Wei-Ping Tien (4447369)
- Gareth Lim (4447366)
- Gladys Yeo (113330)
- Suzanna Chiang (4447363)
- Chee-Seng Chong (440652)
- Lee-Ching Ng (221663)
- Hapuarachchige Hapuarachchi (3802921)
Publication date
September 2017
DOIAbstract
Melting curve analysis demonstrating the non-specific amplification by primer combinations F + R3, F + R4 and F3 + R4 in the presence of mosquito-derived RNA and specificity of F + R3 in a panel of flavivirus and alphavirus RNA. a. Melting peak analysis for the F + R4 primer pair b. Melting peak analysis for the F3 + R4 primer pair c. Melting peak analysis for the F + R3 primer pair. d. Melting peak analysis for the F + R3 primer pairs within a panel of flavivirus and alphavirus RNA. Abbreviation: NTC, Negative control. (TIFF 2225 kb
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