The effect of SOX14wt and SOX14DN1 on p53 protein in HeLa cells.

<p><b>A</b>- Western blot analysis of p53 protein expression in HeLa cells transfected with either empty vector (mock), SOX14wt or SOX14DN1. GAPDH was used as the loading control. Quantification of the effect of SOX14 overexpression on p53 protein level is presented in the graph. Amounts of p53 protein in transfected cells were calculated as a percentage of the p53 in mock transfected cells, which was set as 100%. Data are given as the means ± SEM of three independent transfection experiments. Mean values were compared with Student's t-test and. P- values calculated, *p ≤ 0.05. <b>B</b>—The effect of SOX14 overexpression on p53 protein level detected by immunocytochemistry. Cells were transfected with empty vector (mock), SOX14wt or SOX14DN1. Cell nuclei were counterstained with DAPI. Scale bar: 20 μm.