The mF2Pal₁₆ pepducin generated from Fpr2 inhibits the activity of Fpr2 in mouse neutrophils.

<p><b>A)</b> WT mouse neutrophils (5 x 10⁴ cells) were pre-incubated without (solid line) or with mF2Pal₁₆ (500 nM) for 5 min before stimulation with the peptide PSMα2 (10 nM, indicated by the arrow) and the release of superoxide anions was continuously measured. <b>Inset:</b> The fMIFL response (10 nM) in the presence (dashed line) or absence (solid line) of mF2Pal₁₆ (1 μM). <b>B)</b> The percent of inhibition on the response induced by PSMα2 (10 nM), F2M2 (150 nM), F2Pal₁₆ (500 nM) and fMIFL (10 nM) was calculated from the each individual agonist peak response in the absence of mF2Pal₁₆ (500 nM, mean ± SD) from three independent experiments with individual mouse.