Reducing cell wall feruloylation by expression of a fungal ferulic acid esterase in <i>Festuca arundinacea</i> modifies plant growth, leaf morphology and the turnover of cell wall arabinoxylans - Fig 9

<p>Ester + ether linked HCAs (A-C) and ester linked HCAs (D-F) in cell wall AIR isolated from 10mm segments along 14–16 cm leaves starting from the elongation zone of control and apoplast (T27 and T27R) or Golgi (T28 and T29) FAE expressing plants. <i>p-</i>coumaric acid (A, D), ferulate monomers (B, E), and total ferulate dimers (C, F). Leaf sections (120–130) were pooled by location along the leaf blade for each of 2 to 3 replicates per line. Ferulate monomers = trans-ferulic + cis-ferulic acid. Ferulate dimers = 8-0-4’-diferulate + 5–5’diferulate + 8–5 cyclic diferulate (benzo form) + 8–5’-diferulate + an unknown ferulate dimer. Mean ± SEM (n = 4–6 from 2 independent FAE expressing plants).