Chromosomal integration of pINV occurs <i>via</i> distinct copies of IS<i>1294</i>.

<p>(A) Agarose gel electrophoresis of pINV DNA purified from nine independent CR^- colonies (lanes 1–9) emerging from native <i>mvp</i>^WT at 21°C that retained all the virulence-related genes tested by multiplex PCR. Lanes: (lane labelled M) M90T, (B) BS176, (D) T3SS PAI-deleted M90T. (B) Schematic representation of chromosomal pINV integration in isolate 4 (left) and isolate 6 (right). The chromosomal and plasmid origins of replication are shown in yellow. ORFs are shown with the same colour coding as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007014#pgen.1007014.g002" target="_blank">Fig 2</a>.