Effects of α-syn expression on 20/26S proteasomal activity in living SH-SY5Y cells.

<p>A cellular 20/26S proteasome assay implementing SH-SY5Y cells that stably express wild-type or A53T α-syn as well as a mock control was performed to assess the effect of the presence of α-syn on proteasomal activity on a neuronal background. (A) Immunocytochemistry confirms comparable levels of α-syn expression in wild-type- and A53T α-syn expressing SH-SY5Y cells indicated by red fluorescence. (B) Both expression of wild-type and A53T α-syn results in decreased proteasomal activity as indicated by decreased relative fluorescence intensity (RFU) in this setting. Time-dependent group differences were analyzed using a two-way ANOVA approach with repeated measurements, wild-type α-syn vs control, p<0.001 and α-syn A53T vs control, p<0.001, depicted are the mean +/- SD, n = 3.