<i>In vitro</i> replication of SHIVs expressing adapted 1054 Env proteins.
- Gregory Q. Del Prete (542636)
- Brandon F. Keele (50342)
- Jeannine Fode (4459183)
- Keyur Thummar (4495870)
- Adrienne E. Swanstrom (4459192)
- Anthony Rodriguez (4459186)
- Alice Raymond (4459195)
- Jacob D. Estes (194109)
- Celia C. LaBranche (172138)
- David C. Montefiori (108747)
- Vineet N. KewalRamani (138643)
- Jeffrey D. Lifson (140183)
- Paul D. Bieniasz (215716)
- Theodora Hatziioannou (220814)
Publication date
September 2017
DOIAbstract
<p>Replication of SHIVs expressing the parental or adapted 1054 Env proteins in huPBMC, rhPBMC and rhesus 221 immortalized T cells. SIV<sub>MAC</sub> was used as control. Individual SHIV stocks were normalized for RT and 100pg RT of each virus was used per 1x10<sup>5</sup> activated hu/rhPBMC. For 221 cells 50pg RT of each virus was used per 1x10<sup>5</sup> cells. Replication was measured by RT in samples collected at the indicated times post-inoculation.</p
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