Quantitative real-time PCR and western analysis of ICAM-1 and VCAM-1 expression in irradiated bEnd.3 cells.
- Newsha Raoufi-Rad (4471432)
- Lucinda S. McRobb (4471423)
- Vivienne S. Lee (4471435)
- David Bervini (4471420)
- Michael Grace (578587)
- Jaysree Ukath (4471414)
- Joshua Mchattan (4471429)
- Varun K. A. Sreenivasan (412532)
- T. T. Hong Duong (4471417)
- Zhenjun Zhao (2576056)
- Marcus A. Stoodley (4471426)
Publication date
September 2017
DOIAbstract
<p>qRT-PCR analysis of ICAM-1 (A) and VCAM-1 (E) gene expression, n = 4 independent experiments. Representative western blots (time course) of ICAM-1 (B) and VCAM-1 (F) protein at 25 Gy. Representative western blots (dose response) of ICAM-1 expression (120 h) (C) and VCAM-1 expression (72 h) (G) post-irradiation. ICAM-1 (D) and VCAM-1 (H) protein expression quantitated using Image J, n = 4. Values are mean ± SEM. Data were normalized to GAPDH (westerns) or HPRT (qRT-PCR).</p
Add to ORKG