Differential expression of PD-1 by donor CD4+ and CD8+ T cells in spleen and BM of allo-HSCT recipients.

<p>A and B. Absolute numbers of donor spleen-derived (CD45.1+ gated) CD4+ and CD8+ T cells harvested from spleen and BM, respectively on day 10, 25 and 103 after transplant of B6 → CB6F1 allo-HSCT (filled bars) and B6 → B6 syn-HSCT (open bars) recipients. C and D represent the numbers of PD-1 expressing donor spleen-derived CD4+ and CD8+ gated T cells, respectively, harvested from the spleen and BM of allo-HSCT (solid lines) and syn-HSCT (dashed lines) recipients. Gray filled histograms are isotype controls. E and F represent the absolute numbers of donor spleen-derived PD-1 expressing CD4+ and CD8+ T cells per spleen, respectively after allo-HSCT (filled bars) and syn-HSCT (open bars) recipients. G and H represent the kinetics of absolute numbers of donor spleen-derived CD4+ and CD8+ T cells per BM, respectively, after allo-HSCT (filled bars) or syn-HSCT (open bars). The symbols “*” and “**”represent the <i>p</i> values <0.05 and <0.005, respectively, Students <i>t</i>-Test. The data are the representative of two independent experiments. Five mice were used per group in each time point.